2009 Sep;80(6):327-32. doi: 10.1016/j.fitote.2009.04.002. Epub 2009 Apr 14.
Flavonoid-induced autophagy in hormone sensitive breast cancer cells.
Abstract
The activity of 8-prenylapigenin (8-PA) and its 3′-methoxylated analogue isocannflavin B (IsoB) was investigated in estrogen-dependent T47-D and estrogen-independent MDA-MB-231 human breast cancer cell lines. 8-PA showed a biphasic effect on T47-D cell proliferation, while no significant effect was observed on MDA-MB-231 cells. Conversely, IsoB exhibited only an inhibitory effect on T47-D cell proliferation, accompanied by the appearance of an intense intracytoplasmic vacuolization of autophagic origin. Moreover, biochemical analysis showed that IsoB reduced Akt phosphorylation and p21(Cip1) expression in T47-D cells. These data show that the prenylflavone moiety is a versatile platform for the induction and modulation of bioactivity.
- PMID:
- 19371773
- [PubMed – indexed for MEDLINE]
Publication Types, MeSH Terms, Substances
Publication Types
MeSH Terms
- Antineoplastic Agents, Phytogenic/pharmacology*
- Antineoplastic Agents, Phytogenic/therapeutic use
- Autophagy/drug effects*
- Breast Neoplasms/drug therapy*
- Breast Neoplasms/pathology
- Cannabis
- Cell Line, Tumor
- Cell Proliferation/drug effects
- Cyclin-Dependent Kinase Inhibitor p21/metabolism
- Cytoplasm/drug effects
- Dose-Response Relationship, Drug
- Estrogens/metabolism*
- Female
- Flavanones/pharmacology*
- Flavones/pharmacology*
- Flavonoids/pharmacology*
- Flavonoids/therapeutic use
- Humans
- Humulus
- Phosphorylation
- Phytoestrogens/pharmacology
- Phytoestrogens/therapeutic use
- Phytotherapy*
- Plant Extracts/pharmacology*
- Plant Extracts/therapeutic use
- Prenylation
- Proto-Oncogene Proteins c-akt/metabolism
- Signal Transduction/drug effects
- Vacuoles/drug effects
Substances
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Full Text Sources
Medical
Graphical abstract
Estrogenic activity of 8-prenylnaringenin (8-PN, 1) is well documented. Here we investigate the biological activity of the prenylated flavone 8-prenylapigenin (8-PA, 2b) and its 3′-methoxylated analogue isocannflavin B (IsoB, 2a).
Keywords
- 8-Prenylapigenin;
- 8-Prenylnaringenin;
- Isocannflavin B;
- Breast cancer cells;
- Cell signalling;
- Autophagy
- Corresponding author. Tel.: +39 0321 375820; fax: +39 0321 375821.
Copyright © 2009 Elsevier B.V. All rights reserved.
